Purity: >95 % as determined by SDS-PAGE and SEC-HPLC
Tag: C-terminal mFc-tag
Endotoxin: <1.0 EU per μg protein as determined by the LAL method.
Activity: Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind 2019-nCoV Spike Protein (RBD, mFc Tag),the EC50 of 2019-nCoV Spike Protein (RBD, mFc Tag) is 15-50ng/mL
Predicted Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) consists of 457 amino acids and predicts a molecular mass of 51.5 kDa
2019-nCov amino acids: Arg319 – Phe541 (Gene Accession Number: YP_009724390.1)
Expressed Host: HEK293 Cells
Predicted N Terminal: Arg
Formulation: Lyophilized from sterile PBS, pH 7.4.Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Lyophilized proteins are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Regulatory/Restrictions: For Research Use Only.