100 μg
SARS-CoV-2 (2019-nCov) Spike Protein (RBD)
A DNA sequence coding the NCP-CoV(2019-nCoV) Spike Protein (RBD) was expressed with the Fc region of mouse IgG1 at the C-terminus.
Predicted Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S2 ECD, His tag) consists of 539 amino acids and predicts a molecular mass of 59.37 kDa.
2019-nCov amino acids: Arg319 – Phe541 (Gene Accession Number: YP_009724390.1)
Purity: >95 % as determined by SDS-PAGE and SEC-HPLC
Tag: C-terminal mFc-tag
Endotoxin: <1.0 EU per μg protein as determined by the LAL method.
Activity: Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind 2019-nCoV Spike Protein (RBD, mFc Tag),the EC50 of 2019-nCoV Spike Protein (RBD, mFc Tag) is 15-50ng/mL
Predicted Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) consists of 457 amino acids and predicts a molecular mass of 51.5 kDa
2019-nCov amino acids: Arg319 – Phe541 (Gene Accession Number: YP_009724390.1)
Expressed Host: HEK293 Cells
Species: 2019-nCoV
Predicted N Terminal: Arg
Formulation: Lyophilized from sterile PBS, pH 7.4.Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping:
Lyophilized proteins are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Regulatory/Restrictions: For Research Use Only.
The coronavirus Spike protein (S) is a large oligomeric transmembrane protein that mediates coronavirus entry into host cells. It contains S1 and S2 two subunits. Spike S1 mainly contains a receptor binding domain (RBD) that recognizes a variety of host cell surface receptors. S2 contains basic elements responsible for the membrane fusion. The coronavirus first binds to a receptor on the host cell surface through Spike S1 subunit, and then fuses viral and host membranes through Spike S2 subunit.