SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) Polyclonal Antibody

£458.00

100 μl

SKU: BSV-COV-AB-04

SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) Polyclonal Antibody

Purified rabbit polyclonal antibody (IgG) recognising full-length recombinant Nucleocapsid Protein from SARS-CoV-2 (COVID-19).

Concentration: 1.01 mg/ml

Clonality: Polyclonal

References:
Saccon E, Chen X, Mikaeloff F, et al. Cell-type-resolved quantitative proteomics map of interferon response against SARS-CoV-2. iScience. 2021;24(5):102420.

Form in stock: Liquid – 1.01 mg/mL.
Host: Rabbit
Immunogen: Full length SARS-CoV-2 (COVID-19) Recombinant Nucleocapsid Protein.
Clonality: Polyclonal
Isotype: IgG
Buffer: 1xPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Purity: Purified by antigen-affinity chromatography.
Conjugation: Unconjugated
Alternative names: 2019-nCoV Nucleocapsid, SARS-CoV-2 Nucleocapsid, COVID-19 Nucleocapsid, 2019-nCoV NP, SARS-CoV-2 NP, COVID-19 NP.
Shipping: Shipped on Dry Ice.
Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For
long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Regulatory/ Restrictions: For in vitro Research Use Only.

Application: Suggested Dilution
Western Blot: 1:1000 – 1:10000
ICC/IF: 1:100-1:1000
IHC-P: 1:100-1:1000
IHC-Fr: Assay dependent
FACS: Assay dependent
IP: 1:100-1:5000
ELISA: Assay dependent
sELISA: Assay dependent

Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Sandwitch ELISA detection of recombinant full-length SARS-CoV-2 nucleocapsid protein using BSV-COV-AB-03/BSV-COV-AB-04 as capture antibody at concentration of 5 μg/ml and a detection antibody at concentration of 1 μg/ml. Mouse IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.
Dilution started at: 1:10000
Western Blot
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody BSV-COV-AB-03/BSV-COV-AB-04 diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SARS-CoV-2 (COVID-19) nucleocapsid antibody (BSV-COV-AB-03/ BSV-COV-AB-04) detects SARS-CoV-2 (COVID-19) nucleocapsid by flow cytometry analysis.
Sample: Vero E6 cells infected with SARS-CoV-2.
Black: Uninfected Vero E6 cells was used as a control.
Red: SARS-CoV-2 (COVID-19) nucleocapsid antibody (BSV-COV-AB-03/ BSV-COV-AB-04) dilution: 1:100.
SARS-CoV-2 (COVID-19) nucleocapsid antibody detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (BSV-COV-AB-03/ BSV-COV-AB-04) diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SARS-CoV-2 (COVID-19) nucleocapsid antibody detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (BSV-COV-AB-03/ BSV-COV-AB-04) diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (BSV-COV-AB-03/ BSV-COV-AB-04) at 1 μg/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.