500 μg
SARS-CoV-2 Nucleocapsid Protein
Full length SARS-CoV-2 (COVID-19) nucleocapsid protein expressed in E.coli cells.
Predicted Molecular Mass: The SARS COV-2 Nucleocapsid Protein consists of 431 amino acids and predicts a molecular mass of 46 kDa.
2019-nCoV Amino Acids: 1-419 aa (Accession No. YP_009724397.2)
Form: Liquid
Tag: His-tag
Expression system: E. coli
Buffer: PBS
Molecular weight: 46 kDa
Purification: > 90%
Shipping: Liquid Protein- shipped on Dry Ice.
Storage: Store at +2°C to +8°C or -20°C. Repeated freezing-thaw cycles should be avoided.
Regulatory/ Restrictions: For in vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Coronaviruses have a positive-sense RNA genome with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express Nucleocapsid (N) protein.
The N protein is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. Besides
1. interacts with the viral membrane protein during viral assembly
2. assists in RNA synthesis and folding
3. plays a role in virus budding
4. affects host cell responses, including cell cycle and translation.
Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. It is a highly immunogenic phosphoprotein, also implicated in viral genome replication, and in modulating cell signalling pathways. It is chosen as a diagnostic tool, due to the conservation of N protein sequence and its strong immunogenicity.