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SARS-CoV-2 Nucleocapsid Protein, recombinant CK 6404-b (1 mg)


1 mg

SKU: CK 6404-b

2019 nCoV/COVID19 Nucleocapsid Recombinant Protein (N)

Full-length nucleocapsid protein of SARS-CoV-2 (COVID-19) expressed in E. coli. Contains 6x His tag located at the C terminal.

Predicted Molecular Mass: The SARS-CoV-2 (COVID19) Recombinant Protein (N) predicts a molecular mass at 48 kDa.

2019 nCoV Amino Acids: 1-419 aa (Accession No.YP_009724397)

Applications: Potentially suitable for ELISA or lateral flow type assay.
Purity: 95 % by SDS-PAGE.
Presentation: 0.025% sodium azide
Endotoxin: Endotoxin level is < 0.1 ng/μg of protein (<1 EU/μg)

Expressed Host: E. Coli
Species: 2019-nCoV.
Predicted Molecular Weight: The SARS-CoV-2 Nucleocapsid Recombinant Protein (N) predicts a molecular mass at 48 kDa.
Formulation: Frozen liquid in PBS and 25mM K2CO3 with preservatives.
Shipping: Liquid Protein- Shipped on Dry Ice.
Storage: ≤-70 °C. Avoid repeated freeze-thaw.
Regulatory/Restrictions: For Research Use Only.

Coronaviruses have a positive-sense RNA genome with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express Nucleocapsid (N) protein.

The N protein is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. Besides
1. interacts with the viral membrane protein during viral assembly
2. assists in RNA synthesis and folding
3. plays a role in virus budding
4. affects host cell responses, including cell cycle and translation.

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. It is a highly immunogenic phosphoprotein, also implicated in viral genome replication, and in modulating cell signalling pathways. It is chosen as a diagnostic tool, due to the conservation of N protein sequence and its strong immunogenicity.