SARS-CoV-2 Nucleoprotein, His-Tag (E.coli) (100 μg)

£633.00

100 μg

SKU: BSV-COV-PR-22

SARS-CoV-2 Nucleoprotein, His-Tag (E. coli)

Recombinant SARS-CoV-2 Nucleoprotein, His-Tag (E.coli) was expressed and purified from E.coli as full-length nucleoprotein (amino acids 1-419), with a C-terminal 6xHis-tag.

Predicted Molecular Mass: SARS-CoV-2 Nucleoprotein, His-Tag (E.coli) consists of amino acids 1 – 419 of the full-length SARS-CoV-2 Nucleoprotein and predicts a molecular mass of 48 kDa.
2019-nCoV Amino Acids: 1 – 419 of the full-length SARS-CoV-2 Nucleoprotein (Accession Number: MN908947)

Purity: >95 % by SDS-PAGE.
Presentation: Liquid
Tag: His-tag
Concentration: 2.4 mg/ml
Expressed Host: E.coli
Species: 2019-nCoV
Predicted Molecular Mass: SARS-CoV-2 Nucleoprotein, His-Tag (E.coli) consists of amino acids 1 – 419 of the full-length SARS-CoV-2 nucleoprotein and predicts a molecular mass of 48 kDa.
2019-nCoV Amino Acids: 1 – 419 of the full-length SARS-CoV-2 nucleoprotein (Gene Accession Number: MN908947)
Formulation: Recombinant protein stored in 25mM Tris base, 10mM K2CO3, pH 9.2-9.4.
Endotoxin: Endotoxin level is < 0.1 ng/μg of protein (<1 EU/μg).
Shipping: Liquid Protein- Shipped on Dry Ice.
Storage: Store the protein at –20°C. For most favourable performance, avoid repeated handling and multiple freeze/thaw cycles.
Regulatory/Restrictions: For Research Use Only.

Coronaviruses have a positive-sense RNA genome with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express Nucleocapsid (N) protein.

The N protein is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. Besides
1. interacts with the viral membrane protein during viral assembly
2. assists in RNA synthesis and folding
3. plays a role in virus budding
4. affects host cell responses, including cell cycle and translation.

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. It is a highly immunogenic phosphoprotein, also implicated in viral genome replication, and in modulating cell signalling pathways. It is chosen as a diagnostic tool, due to the conservation of N protein sequence and its strong immunogenicity.