SARS-CoV-2 Nucleoprotein, His-Tag (E.coli) (500 μg)

Purity: >80 % by SDS-PAGE.
Tag: His-tag
Activity: Testing in process
Concentration: 2.4 mg/ml
Expressed Host: E.coli
Species: SARS
Predicted Molecular Mass: SARS-CoV-2 Nucleoprotein, His-Tag (E.coli) consists of amino acids 1 – 419 of the full-length SARS-CoV-2 nucleoprotein and predicts a molecular mass of 48 kDa.
2019-nCoV Amino Acids: 1 – 419 of the full-length SARS-CoV-2 nucleoprotein
Formulation: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Endotoxin: Endotoxin level is < 0.1 ng/μg of protein (<1 EU/μg). Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage: Store product at –20°C. For most favourable performance, avoid repeated handling and multiple freeze/thaw cycles.
Regulatory/Restrictions: For Research Use Only.

SARS-CoV-2 Recombinant Nucleoprotein, His-Tag (E.coli) Datasheet

Coronaviruses have a positive-sense RNA genome with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express Nucleocapsid (N) protein.

The N protein is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. Besides
1. interacts with the viral membrane protein during viral assembly
2. assists in RNA synthesis and folding
3. plays a role in virus budding
4. affects host cell responses, including cell cycle and translation.

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. It is a highly immunogenic phosphoprotein, also implicated in viral genome replication, and in modulating cell signalling pathways. It is chosen as a diagnostic tool, due to the conservation of N protein sequence and its strong immunogenicity.