SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid Recombinant Protein His-tag (100 µg)


100 µg


Source: DNA sequence encoding extracellular fragment[1-419] of COVID-19 Nucleocapsid protein (B.1.1.529/Omicron) variant (P13L, E31 R32S33 deletion, R203K, G204R) including a C terminal polyHis tag was expressed in HEK cells.
Molecular weight: Recombinant 2019-nCoV nucleocapsid protein B.1.1.529 encodes the COV2 Nucleocapsid glycoprotein. The recombinant Nucleocapsid-His protein migrates as 70 kDa under reducing conditions in SDS-PAGE due to glycosylation.
Purity: >95%, as determined by SEC-HPLC.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation: Recombinant nCoV-2019 Nucleocapsid protein was lyophilized from 0.2 µm filtered PBS, pH 7.4.
Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Shipping: Lyophilized protein- shipped at ambient temperature.
Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Repeated freeze-thaw cycles should be avoided.
Usage: This product is for Research Use Only. It may not be used for therapeutics or diagnostic purposes.

Coronaviruses have a positive-sense RNA genome with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express Nucleocapsid (N) protein.

The N protein is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. Besides
1. interacts with the viral membrane protein during viral assembly
2. assists in RNA synthesis and folding
3. plays a role in virus budding
4. affects host cell responses, including cell cycle and translation.

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. It is a highly immunogenic phosphoprotein, also implicated in viral genome replication, and in modulating cell signalling pathways. It is chosen as a diagnostic tool, due to the conservation of N protein sequence and its strong immunogenicity.