1 mg
SARS-CoV-2 S1 Receptor-Binding Domain (S1-RBD), His-Tag (CHO)
Recombinant spike glycoprotein Receptor-Binding Domain (RBD) from SARS-CoV-2, Wuhan-Hu-1-isolate, produced under serum-free conditions in HEK293 cells.
Predicted Molecular Mass: The SARS-CoV-2 S1 Receptor-Binding Domain (S1-RBD), His-Tag (CHO), after glycosylation predicts a molecular mass of 26 kDa. Glycosylated form runs at 40 – 55 kDa in gel electrophoresis.
nCoV Amino Acids: 319-541 aa (Accession No.QHD43416.1 )
Source: Receptor-binding domain (RBD) of SARS-CoV-2 Spike protein S1 (aa 319-541) fused to the C-terminus with a 6xHis-tag.
Molecular weight: Recombinant NCOV S1 encodes the NCOV Spike glycoprotein S1 sequence. This molecule has a calculated mass of approximately 26 kDa. Recombinant S1 migrates due to glycosylation as an approximately 40-55 kDa protein under reducing conditions in SDS-PAGE.
Expression System: Mammalian; CHO
Tag: C-terminal 6 x His-tag.
Purity: >90%, as determined by analytical CGE under reducing conditions.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.001 EU/μg.
Formulation: Liquid, 20mM NaPP, 300mM NaCl pH 7.2.
Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Shipping: The liquid protein is shipped on Dry Ice.
Storage: The liquid protein needs to be stored between 2°C and 8°C upon receipt.
Usage: This product is for Research Use Only. It may not be used for therapeutics or diagnostic purposes.