All viruses undergo fast mutations and adept quickly to the countermeasures that the immune systems creates against them. SARS-CoV-2 of the COVID-19 pandemic is no exception here. During the pandemic multiple mutant strains arose. Spike protein of the mutant strain B.1.1.529, also commonly known as the “Omicron Variant”. It is a full-length protein, which is active in its native trimeric form, that is stabilized in LMNG detergent.
UniProt Number: P0DTC2
WHO Label: Omicron (Variant of Concern)
Mutations that differ from SPIKE Protein: A67V; H69del; V70del; T95I; G142D; V143del; Y144del; Y145del; N211del; L212I; INS 214EPE; G339D; S371L; S373P; S375F; K417N; N440K; G446S; S477N; T478K; E484A; Q493K; G496S; Q498R; N501Y; Y505H; T547K; D614G; H655Y; N679K; P681H; N764K; D796Y; N856K; Q954H; N969K; L981F
Further Modifications: Furin cleavage site “RRAR” mutated to “GSAG”; K986P V987P
Buffer Composition: 20 mM Hepes pH 7.5; 150 mM NaCl, 0.001% LMNG
Molecular Weight (Trimeric): 3 x 142 kDa = 426 kDa