Set of N3 Primers & Probe for SARS-CoV-2 detection (50 qPCR reactions)

3×20µL

SKU: BCC-19nCoVN3_S50

BaseClick provides primers and probes recommended by the Centers for Disease Control and Prevention to researchers for the in vitro detection of the novel coronavirus (2019-nCoV). The primers and probes are designed for the universal detection of SARS-like coronaviruses (N3 assay) and for the specific detection of novel 2019-nCoV (N1 and N2 assays).
RP – all clinical samples should be tested for human RNAse P (RNP) gene to assess specimen quality.
If you are interested in primers and probes to create a Real-Time RT-PCR panel in your lab, follow the panel instructions from the CDC to get more instructions for use.

Catalog No.DescriptionSequence (5’-3’)LengthLabel
BCC-19nCoVN3_F50Forward Primer for 50 qPCR reactionsGGG AGC CTT GAA TAC ACC AAA A22-
BCC-19nCoVN3_R50Reverse Primer for 50 qPCR reactionsTGT AGC ACG ATT GCA GCA TTG21-
BCC-19nCoVN3_P50Probe for 50 qPCR reactionsFAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ124FAM / BHQ1
BCC-19nCoVN3_S50Set of N3 Primers & Probe for 50 qPCR reactionsGGG AGC CTT GAA TAC ACC AAA A
TGT AGC ACG ATT GCA GCA TTG
FAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ1
22
21
24
-
-
FAM / BHQ1
BCC-19nCoVN3_F100Forward Primer for 100 qPCR reactionsGGG AGC CTT GAA TAC ACC AAA A22-
BCC-19nCoVN3_R100Reverse Primer for 100 qPCR reactionsTGT AGC ACG ATT GCA GCA TTG21-
BCC-19nCoVN3_P100Probe for 100 qPCR reactionsFAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ124FAM / BHQ1
BCC-19nCoVN3_S100Set of N3 Primers & Probe for 100 qPCR reactionsGGG AGC CTT GAA TAC ACC AAA A
TGT AGC ACG ATT GCA GCA TTG
FAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ1
22
21
24
-
-
FAM / BHQ1

These sequences are intended to be used for the purposes of respiratory virus surveillance and research. The recipient agrees to use them in compliance with all applicable laws and regulations.

Sequences and lengths:
GGG AGC CTT GAA TAC ACC AAA A (22bp)
TGT AGC ACG ATT GCA GCA TTG (21bp)
FAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ1 (24bp)
Content volume: 3×20µL
Number of reactions: 50
Label: FAM/BHQ1
Shelf life: 12 months unopened after receipt
Storage conditions: -20 °C
Solubility: water, aqueous buffer

How can I extract the viral RNA from the specimens ?
The quality of the assay is largely dependent on the quality of input RNA.
Commercially available extraction procedures that have been shown to generate highly purified RNA when following manufacturer’s recommended procedures for sample extraction include:

  • QIAamp® Viral RNA Mini Kit
  • QIAamp® MinElute Virus Spin Kit or RNeasy® Mini Kit (QIAGEN)
  • EZ1 DSP Virus Kit (QIAGEN)
  • Roche MagNA Pure Compact RNA Isolation Kit
  • Roche MagNA Pure Compact Nucleic Acid Isolation Kit
  • Roche MagNA Pure 96 DNA and Viral NA Small Volume Kit
  • Invitrogen ChargeSwitch® Total RNA Cell Kit.

What are the precautions I need to use ?
Wear appropriate personal protective equipment (e.g. gloves, eye protection) when working with clinical specimens. Specimens processing should be performed in a certified class II biological safety cabinet following biosafety level 2 guidelines.
What kind of specimens can I use for the assay?
Respiratory specimens and sera, e.g. nasopharyngeal aspirates or washes, swabs, bronchioalveolar lavage and sputum can be used for the detection of virus RNA.