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Trimeric SARS-CoV-2 Spike Protein, Full-length (10 mg)

£24,500.00

10 mg

SKU: BSV-COV-PR-35

Full length SARS Cov-2 spike protein

Full length SARS-CoV-2 Spike Protein is single span transmembrane protein which contains the Spike protein amino acids 1-1273. Full length SARS-CoV-2 Spike Protein is manufactured in HEK293 mammalian cells, ensuring the most authentic post-translational modifications.

  • Single span transmembrane membrane protein (aa 1 – 1273)
  • Furin cleavage site RRAR mutated to GSAS
  • Trimerization (3 x 142 kDa)
  • Expressed in HEK293 Expi cells
  • Solubilization and stabilization in LMNG detergent
  • 2-step purification via Rho1D4 tag and size exclusion chromatography in LMNG detergent

Product name: Trimeric SARS CoV-2 Spike Protein, Full-length
Species: 2019-nCoV, COVID-19
Sequence: Full-length sequence (aa 1 – 1273), furin cleavage site “RRAR” mutated to “GSAG”; C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence.
Accession No.: YP_009724390.1 (GenBank), P0DTC2 (UniProt).
Host: Expressed in HEK293 Expi cells.
Applications: ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and Biophysical analysis.
Purity: > 98% by SDS-PAGE gel.
Trimerisation: 3 x 142 kDa. Includes transmembrane domain, a detergent screen was performed.
Purification: 2-step purification via Rho1D4 tag and size exclusion chromatography in LMNG detergent. Confirmed by SDS-PAGE, native gel, Western Blot.
Formulation: Liquid – Purified protein (1 mg/ml) supplied in 20 mM Hepes pH 7.5; 150 mM NaCl, 0.001 % LMNG.
Shipping:The product is liquid protein-shipped with dry ice.
Storage: Upon receipt, unopened vial can be stored at -80°C for over 12 months. Repeated freeze/thaw cycles should be avoided.

The spike (S) glycoprotein of coronaviruses is located on the surface of SARS-CoV-2, commonly known as the Coronavirus. It is known to be essential in the binding of the virus to the host cell at the advent of the infection process. In its native form it is a homo trimer and it is located in the phospholipid membrane of the virus. Hence, to purify S in its native form, the homo trimer needs to be kept intact.

It contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. It’s been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The S protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

The main functions for the Spike protein are summarized as:
Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component is to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.