Viral DNA/RNA Nucleic Acid Mini Kit

£300.00

100 reactions

SKU: 4SDO0023

Viral DNA/RNA Nucleic Acid Mini Kit is an excellent tool for the extraction and purification of viral nucleic acids (DNA and RNA) from viral cell free specimen such as, serum, plasma, body fluid and cell cultured supernatant, and from transport medium of swabs. The extraction method is based on the silica membrane/ chao-tropic salt technology. This entails: efficient lysis of the virus; optimization of binding condition allowing strong interaction of the viral nucleic acid to the binding column’s silica membrane; efficient removal of contamination (including alts, metabolites, nucleases and other components of body fluid) from the silica membranes; and elution of the viral nucleic acid from the silica membrane with a minimum volume of 30 μl RNase free water.

Format: spin column/ silica membrane/ chaotropic salt
Sample size: 140 μl cell-free fluid such as serum, plasma, body fluid and cell cultured supernatant
Operation time: < 20 min
Recovery rate: 80 ~ 90 %
Length of recovery nucleic acid: > 200 bp
Column Binding capacity: 60 μg RNA /column
Elution volume: 40 ~ 50 μl
Column applicability: centrifugation and vacuum

Materials Supplied:

  • VNE buffer
  • Wash buffer 1
  • Wash buffer 2
  • RNAse-free water
  • VNE columns
  • Collection tubes
  • Elution tubes
  • User Manual
  • Shipment and Storage:
    1. Kit components except Carrier RNA should be stored at room temperature (15 – 25 °C).
    2. Carrier RNA should be stored at -20 °C upon receipt.
    3. VNE Buffer should be stored at 4 °C after adding Carrier RNA.