ViralPolish® chromatography media specifically for virus purification; Comprising dual layer agarose beads with an inert external shell with tightly controlled pore size and internal polyfunctional ligands for rapid high capacity binding of impurities.

This media is ideal for rapid clean-up, either as a first step or as a second step, to purify virus particles.

Large particles, such as viruses, are excluded from the beads. The beads can be packed in a column where the virus will pass through the column bed and collected in the flow through fraction, whilst impurities will be captured within the beads. This affords a very gentle purification process resulting in efficient clean up and high recovery of active virus particles.

The beads can also be used in batch mode; when added to a flask of virus containing media the beads will selectively allow small molecules to enter and be retained, whilst the virus is excluded and remains in the external media.

A typical purification strategy for virus would be to use ViralPolish® as an initial step, to clean up the sample, prior to binding and elution, typically from an anion exchange media designed for virus purification such as SepFast Supor Q.

Alternatively, very dilute samples can be purified by first passing through a SepFast Supor Q anion exchange column to concentrate the sample followed by passing through ViralPolish column to remove residual impurities.

ViralPolish® is available in three different outer shell pore sizes, and two different impurity binding chemistries (see table below).

ViralPolish 5000A and 5000B

5000A Application: Good for lentivirus, adeno virus, VLPs and other viruses of large sizes.

5000B Application: AAV may be purified if very high molecular-weight impurity (e.g. truncated AAV or empty capsid) need be removed.

Molecular weight cut-off: 5000 kDa

ViralPolish 700A and 700B

Application: Good for AAVs, other viruses
and VLPs of similar sizes, plasmids etc

Molecular weight cut-off: 700 kDa

ViralPolish 400A and 400B

Application: Good for certain vaccine antigens, large proteins etc.

Molecular weight cut-off: 400 kDa