Trimeric SARS CoV-2 Spike Antigen, Full-length
Coronavirus enters the host cell mediated by the transmembrane spike glycoprotein binding to the host ACE2 protein. This spike (S) protein is comprised of homotrimers with two functional subunits (S1 and S2). The trimeric protein also contains an S2’ site which is cleaved by host proteases just upstream of the fusion peptide, activating membrane fusion. The structure of the SARS-CoV-2 protein has been solved and shown to bind very tightly to the human ACE2 protein. Further investigation of the relationship between this viral and host protein will provide insight into therapeutic and diagnostic development for COVID-19.
The Only Native, Wild-type Trimeric SARS CoV-2 Spike Antigen on the Market
Full length SARS-CoV-2 Spike Protein is a single span transmembrane protein which contains the Spike protein amino acids 1-1273. Full length SARS-CoV-2 Spike Protein is manufactured in HEK293 mammalian cells, ensuring the most authentic post-translational modifications.
- The conformation is identical to the membrane bound trimeric spike protein on the viral envelope.
- Single span transmembrane membrane protein (aa 1 – 1273)
- Furin cleavage site RRAR mutated to GSAS
- Supplied in its native state as a Trimer (3 x 142 kDa)
- Expressed in HEK293 Expi cells
- C-terminal Rho1D4 tag for affinity purification
- Solubilization and stabilization in LMNG detergent
- 2-step purification via Rho1D4 tag and size exclusion chromatography in LMNG detergent
Product name: Trimeric SARS CoV-2 Spike Protein, Full-length
Species: 2019-nCoV, COVID-19
Sequence: Full-length sequence (aa 1 – 1273), furin cleavage site “RRAR” mutated to “GSAG”; C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Accession No.: YP_009724390.1 (GenBank), P0DTC2 (UniProt).
Tag: C-terminal Rho1D4 tag for affinity purification
Host: Expressed in HEK293 Expi cells
Applications: ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and Biophysical analyses
Purity: > 98% by SDS-PAGE gel
Trimerisation: 3 x 142 kDa. Includes transmembrane domain, a detergent screen was performed.
Purification: 2-step purification via Rho1D4 tag and size exclusion chromatography in LMNG detergent. Confirmed by SDS-PAGE, native gel, WB.
Formulation: Liquid – Purified protein (1 mg/ml) supplied in 20 mM Hepes pH 7.5; 150 mM NaCl, 0.001 % LMNG
Storage: The product is shipped with dry ice. Upon receipt, unopened vial can be stored at -80°C for over 12 months. Avoid repeated freeze/thaw cycles.